《植物生理学报》 2014, 50(10): 1546-1554

亚精胺调控菊花不定根发生的生理机制

徐东花, 孙霞, 孙宪芝*, 徐璐

山东农业大学园艺科学与工程学院, 作物生物学国家重点实验室, 山东泰安271018

通信作者：孙宪芝；E-mail: sunxianzhi@126.com；Tel: 0538-8246139

摘　要：

以菊花(Dendranthema morifolium)品种‘神马’为试验材料, 研究外源亚精胺(spermidine, Spd)及其抑制剂二环己胺(dicyclohexyl amine, DCHA)对不定根发生及相关生理生化指标的影响。结果显示, 外源Spd处理可以比对照提前2 d生根, 并且对生根和种苗质量提高有显著效果, 增加了扦插菊花叶片叶绿素、可溶性糖、淀粉和可溶性蛋白的积累, 并且显著提高了茎基部IAA的含量以及IAA/ABA和IAA/ZR的比值, 降低了ABA、ZR、JA、GA含量, 同时提高茎基部POD和PPO活性; DCHA对生根和种苗质量有抑制作用, 并且生理指标均表现出不同程度的抑制作用; DCHA处理完再用Spd处理的插穗可以缓解DCHA的抑制。结果表明: 一定浓度的Spd通过影响叶绿素、营养物质、内源激素及相关酶活性, 影响菊花不定根的形成。

关键词：菊花; 亚精胺; 扦插生根; 营养物质; 内源激素; 氧化酶

收稿：2014-07-28   修定：2014-09-07

资助：国家自然科学基金(31101564)。

Physiological Mechanism of Spermidine Regulation on Formation of Adventitious Roots in Chrysanthemum

XU Dong-Hua, SUN Xia, SUN Xian-Zhi*, XU Lu

State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, Shandong 271018, China

Corresponding author: SUN Xian-Zhi; E-mail: sunxianzhi@126.com; Tel: 0538-8246139

Abstract:

Chrysanthemum ‘Jinba’ was used as material to study the influence of exogenous spermidine (Spd) and its inhibitor dicyclohexyl amine (DCHA) on formation of adventitious roots and relative physiological and biochemical parameters. Results showed that, rooting was advanced for 2 days with the treatment of exogenous Spd compared with CK, and Spd significantly promoted rooting and seeding quality. Spd could increase the accumulation of chlorophyll, soluble sugar, starch and soluble protein and increased the activities of POD and PPO compared with those of the control. Plant hormones were specially affected by exogenous Spd application. Compared to the control, the content of IAA and the ratios of IAA/ABA and IAA/ZR in the base of steams were higher with the Spd treatment. Spd reduced the contents of ABA, ZR, JA and GA. DCHA had the opposite results. The rooting ability induced by submerged in DCHA, and then submerged in Spd was similar to the control. The results illustrate that, polyamine may influence the contents of chlorophyll, nutrients, hormones and related enzyme activities, thereby regulate adventitious rooting of chrysanthemum.

Key words: chrysanthemum; spermidine; rooting of softwood cuttings; nutrients; endogenous hormones; oxidases